Chemical sclerosis of the gallbladder was attempted using 99.9% ethanol in 13 rabbits at laparotomy. This study was performed to observe the obliteration and fibrosis of the gallbladder after exposure to chemical sclerosant without operative
cholecystectomy. The proximal cystic duct was occluded by vascular clamps, and then 99.9% ethanol was injected to the gallbladder, followed by aspiration of ethanol after 10min (4 rabbits), 20min(5 rabbits). Biopsies of the gallbladder were
performed
after 1 weeks in 9 rabbits(twice for each rabbits), and after 3 weeks in remaining 4 rabbits. The gallbladder specimens were examined grossly and microscopically to observe the degree and extent of necrosis and fibrosis related to the
ethanol-expousre
time and the interval between the time of the ethanol-expousre and biopsy.
@ES The results were as follows:
@EN 1. Cystic duct occlusions were observed at 1 week after ethanol-exposure in all rabbits, and there were no gross changes of the liver and the common bile duct.
2. The specimen of the liver showed no remarkable microscopic change, and the titers of liver function test (AST, ALT and r-GTP) were in normal limit in all rabbits after ethanol-exposure.
3. Microscopic examination of the wall of the gallbladder after 2 week showed ischemic necrosis of entire layer in all rabbits, but inflammation or fibrosis was not observed.
4. After 2 weeks, the serosal layer of the gallblasdder showed fibrosis with infiltration of fibroblasts in all rabbits, and the fibrosis was more progressed after 3weeks with proliferating fibroblasts.
|